PMID- 38224793 OWN - NLM STAT- MEDLINE DCOM- 20240214 LR - 20240214 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) VI - 324 DP - 2024 Apr 24 TI - Intranasal administration of the essential oil from Perillae Folium ameliorates social defeat stress-induced behavioral impairments in mice. PG - 117775 LID - S0378-8741(24)00074-6 [pii] LID - 10.1016/j.jep.2024.117775 [doi] AB - ETHNOPHARMACOLOGICAL RELEVANCE: Perillae Folium, the leaves and twigs of Perilla frutescens (L.) Britton, has been included in many traditional Chinese medicine herbal formulas to treat depression. However, the precise antidepressant mechanism of the essential oil from Perillae Folium (PFEO) has not been fully investigated. AIM OF THE STUDY: To assess the effects and potential mechanisms of PFEO on depression using animal models and network pharmacology analysis. MATERIALS AND METHODS: PFEO was intranasally administered to a mouse model of social defeat stress (SDS). The antidepressant effects of PFEO on SDS-induced mice were evaluated using behavioral tests. Enzyme-linked immunosorbent assay (ELISA) and western blot were performed to measure the levels of depression-related biomarkers in the hippocampus and serum of the mice. The chemical compounds of PFEO were determined using gas chromatography-mass spectrometry (GC-MS). Network pharmacology and molecular docking analyses were conducted to investigate the potential bioactive components of PFEO and the mechanisms underlying the antidepressant effects. To validate the mechanisms of the bioactive compounds, in vitro models using PC12 and BV2 cells were established and the blood-brain barrier (BBB) permeability was evaluated. RESULTS: The intranasal administration of PFEO suppressed SDS-induced depression in mice by increasing the time spent in the social zone and the social interactions in the social interaction test and by decreasing the immobility time in the tail suspension and forced swimming tests. Moreover, the PFEO treatment reduced the SDS-induced anxiety-like behavior, as inferred from the increased activity in the central zone observed in the open field test and in the open arms observed in the elevated plus maze test. PFEO administration recovered the SDS-induced decrease in the levels of 5-HT, NE, gamma-aminobutyric acid (GABA), and p-ERK in the hippocampus of mice. Furthermore, the increased serum corticosterone level was also attenuated by the PFEO treatment. A total of 21 volatile compounds were detected in PFEO using GC-MS, among which elemicin (15.52%), apiol (15.16%), and perillaldehyde (12.79%) were the most abundant ones. The PFEO compounds targeted 32 depression-associated genes, which were mainly related to neural cells and neurotransmission pathways. Molecular docking indicated good binding affinities between the bioactive components of PFEO (apiol, β-caryophyllene, elemicin, and myristicin) and the key targets, including ACHE, IL1B, IL6, MAOB, SLC6A2, SLC6A3, SLC6A4, and tumor necrosis factor. Among the four compounds, β-caryophyllene, elemicin, and myristicin were more effective in reducing neurotoxicity and neuroinflammation. Elemicin showed the highest BBB permeability rate. CONCLUSIONS: This study shows the antidepressant activities of PFEO in an SDS-induced mouse model and suggests its potential mechanisms of action: regulation of the corticosterone levels, hippocampal neurotransmitters, and ERK signaling. Apiol, β-caryophyllene, elemicin, and myristicin may be the main contributors to the observed effects induced by PFEO. Further studies are needed to fully elucidate the underlying mechanisms and the main PFEO bioactive components. CI - Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Nguyen, Ly Thi Huong AU - Nguyen LTH AD - Department of Physiology, Dongguk University College of Korean Medicine, Gyeongju, 38066, Republic of Korea; Department of Pathology, University of Alabama at Birmingham, Birmingham, AL, 35294, USA. Electronic address: lynguyen@dgu.ac.kr. FAU - Nguyen, Nhi Phuc Khanh AU - Nguyen NPK AD - Department of Physiology, Dongguk University College of Korean Medicine, Gyeongju, 38066, Republic of Korea. Electronic address: npkhanhnhi@dgu.ac.kr. FAU - Tran, Khoa Nguyen AU - Tran KN AD - Department of Physiology, Dongguk University College of Korean Medicine, Gyeongju, 38066, Republic of Korea. Electronic address: trannguyen053@dgu.ac.kr. FAU - Shin, Heung-Mook AU - Shin HM AD - Department of Physiology, Dongguk University College of Korean Medicine, Gyeongju, 38066, Republic of Korea. Electronic address: heungmuk@dongguk.ac.kr. FAU - Yang, In-Jun AU - Yang IJ AD - Department of Physiology, Dongguk University College of Korean Medicine, Gyeongju, 38066, Republic of Korea. Electronic address: injuny@dongguk.ac.kr. LA - eng PT - Journal Article DEP - 20240113 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 RN - BHW853AU9H (caryophyllene) RN - 04PD6CT78W (myristicin) RN - 0 (Oils, Volatile) RN - W980KJ009P (Corticosterone) RN - 487-11-6 (elemicin) RN - 0 (Antidepressive Agents) RN - 0 (Polycyclic Sesquiterpenes) RN - 0 (Allylbenzene Derivatives) RN - 0 (Dioxolanes) RN - 01Y4A2QXY0 (Pyrogallol) SB - IM MH - Animals MH - Mice MH - *Depression/drug therapy MH - *Oils, Volatile/pharmacology/therapeutic use MH - Corticosterone MH - Administration, Intranasal MH - Molecular Docking Simulation MH - Social Defeat MH - Antidepressive Agents/pharmacology/therapeutic use MH - Behavior, Animal MH - Hippocampus MH - Disease Models, Animal MH - *Polycyclic Sesquiterpenes MH - *Allylbenzene Derivatives MH - *Dioxolanes MH - Pyrogallol/*analogs & derivatives OTO - NOTNLM OT - Anxiety OT - Depression OT - Essential oil OT - Neurotransmitter OT - Perillae Folium OT - Social defeat stress COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2024/01/16 00:42 MHDA- 2024/02/10 14:49 CRDT- 2024/01/15 19:31 PHST- 2023/11/26 00:00 [received] PHST- 2024/01/08 00:00 [revised] PHST- 2024/01/12 00:00 [accepted] PHST- 2024/02/10 14:49 [medline] PHST- 2024/01/16 00:42 [pubmed] PHST- 2024/01/15 19:31 [entrez] AID - S0378-8741(24)00074-6 [pii] AID - 10.1016/j.jep.2024.117775 [doi] PST - ppublish SO - J Ethnopharmacol. 2024 Apr 24;324:117775. doi: 10.1016/j.jep.2024.117775. Epub 2024 Jan 13. PMID- 38384478 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20240224 IS - 1319-0164 (Print) IS - 2213-7475 (Electronic) IS - 1319-0164 (Linking) VI - 32 IP - 3 DP - 2024 Mar TI - Chemical profile and biological properties of the Piper corcovadense C.DC. essential oil. PG - 101993 LID - 10.1016/j.jsps.2024.101993 [doi] LID - 101993 AB - The essential oil from Piper corcovadense D.DC. (EOPc), an important plant belonging to the Piperaceae family, which is commonly found in the northern region of Brazil and poorly explored scientifically, was used in this study. Thus, the EOPc was characterized chemically by Gas Chromatography/Mass Spectrometry (GC/MS) and the antioxidant and antimicrobial activities and their potential effects on cutaneous melanoma (SK-MEL-28) and healthy peripheral blood mononuclear (PBMC) cells were determined. The major compounds identified in the EOPc were: trans-sesquisabinene hydrate, trans-caryophyllene, β-pinene, trans-β-farnesene, 14-hydroxycaryophyllene, limonene and p-cymene. The EOPc demonstrated antioxidant activity as evaluated by Folin-Ciocalteu reagent (FC) reducing capacity, DPPH, and ABTS methods. The values found were respectively 5.41 ± 0.17 mg GAE mL(-1) (GAE: Gallic acid equivalent), 2.88 ± 0.17 µmol TE mL(-1) (TE: Trolox equivalent) and 6.26 ± 0.02 µmol TE mL(-1). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined for different bacterial strains. The EOPc at a concentration of 2.61 µg mL(-1) exhibited both bactericidal and bacteriostatic properties against Escherichia coli. The EOPc showed potential antitumor activity as it reduced the cell viability of human cutaneous melanoma cells SK-MEL-28. Besides, the EOPc did not exhibit cytotoxic activity against healthy PBMCs, indicating that it does not harm healthy cells at the tested concentrations. The EOPc increased the levels of ROS at concentrations of 250 µg mL(-1). The EOPc also did not stimulate the mobilization of endogenous antioxidant defenses, as assessed by total thiol (PSH) and non-protein thiols (NPSH). Thus, the study suggests that the EOPc has antioxidant and antimicrobial properties due to the presence of specific compounds. It also exhibits antitumor potential against cutaneous melanoma cells while showing no cytotoxicity to healthy PBMCs. It directly influenced ROS levels at the highest tested concentration in the cells, suggesting an antitumor effect related to the intrinsic apoptosis pathway. Nevertheless, while the study has initial findings, the results are promising and indicate an attractive biological potential of P. corcovadense, mainly in human cutaneous melanoma cells. CI - © 2024 The Authors. Published by Elsevier B.V. on behalf of King Saud University. FAU - Henrique Fontoura, Bruno AU - Henrique Fontoura B AD - Department of Chemistry, Postgraduate Program in Chemical and Biochemical Process Technology (PPGTP), Federal Technological University of Paraná, Campus Pato Branco, PO Box 571, CEP 85503-390 PR, Brazil. FAU - Cristina Perin, Ellen AU - Cristina Perin E AD - Department of Chemistry, Postgraduate Program in Chemical and Biochemical Process Technology (PPGTP), Federal Technological University of Paraná, Campus Pato Branco, PO Box 571, CEP 85503-390 PR, Brazil. FAU - Paula Buratto, Ana AU - Paula Buratto A AD - Department of Chemistry, Postgraduate Program in Chemical and Biochemical Process Technology (PPGTP), Federal Technological University of Paraná, Campus Pato Branco, PO Box 571, CEP 85503-390 PR, Brazil. FAU - Francisco Schreiner, Jucemar AU - Francisco Schreiner J AD - Department of Chemistry, Postgraduate Program in Chemical and Biochemical Process Technology (PPGTP), Federal Technological University of Paraná, Campus Pato Branco, PO Box 571, CEP 85503-390 PR, Brazil. FAU - Menezes Cavalcante, Kamyla AU - Menezes Cavalcante K AD - Department of Chemistry, Postgraduate Program in Chemical and Biochemical Process Technology (PPGTP), Federal Technological University of Paraná, Campus Pato Branco, PO Box 571, CEP 85503-390 PR, Brazil. FAU - Dias Teixeira, Sirlei AU - Dias Teixeira S AD - Department of Chemistry, Postgraduate Program in Chemical and Biochemical Process Technology (PPGTP), Federal Technological University of Paraná, Campus Pato Branco, PO Box 571, CEP 85503-390 PR, Brazil. FAU - Manica, Daiane AU - Manica D AD - Postgraduate Program in Biochemistry, Federal University of Santa Catarina, Florianópolis, SC, Brazil. FAU - Antônio Narzetti, Rafael AU - Antônio Narzetti R AD - Postgraduate Program in Biochemistry, Federal University of Santa Catarina, Florianópolis, SC, Brazil. FAU - Bruno da Silva, Gilnei AU - Bruno da Silva G AD - Multicentric Postgraduate Program in Biochemistry and Molecular Biology, State University of Santa Catarina, Lages, SC, Brazil. FAU - Dulce Bagatini, Margarete AU - Dulce Bagatini M AD - Postgraduate Program in Biochemistry, Federal University of Santa Catarina, Florianópolis, SC, Brazil. AD - Postgraduate Program in Biomedical Sciences, Federal University of Fronteira Sul, Chapecó, SC, Brazil. FAU - Luiza Cadorin Oldoni, Tatiane AU - Luiza Cadorin Oldoni T AD - Department of Chemistry, Postgraduate Program in Chemical and Biochemical Process Technology (PPGTP), Federal Technological University of Paraná, Campus Pato Branco, PO Box 571, CEP 85503-390 PR, Brazil. FAU - Teresinha Carpes, Solange AU - Teresinha Carpes S AD - Department of Chemistry, Postgraduate Program in Chemical and Biochemical Process Technology (PPGTP), Federal Technological University of Paraná, Campus Pato Branco, PO Box 571, CEP 85503-390 PR, Brazil. LA - eng PT - Journal Article DEP - 20240212 PL - Saudi Arabia TA - Saudi Pharm J JT - Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society JID - 9705695 PMC - PMC10879029 OTO - NOTNLM OT - Antibacterial OT - Antioxidant OT - Cell viability OT - Essential oil OT - Melanoma cells OT - Terpenes EDAT- 2024/02/22 06:43 MHDA- 2024/02/22 06:44 CRDT- 2024/02/22 03:49 PHST- 2023/08/03 00:00 [received] PHST- 2024/02/11 00:00 [accepted] PHST- 2024/02/22 06:44 [medline] PHST- 2024/02/22 06:43 [pubmed] PHST- 2024/02/22 03:49 [entrez] AID - S1319-0164(24)00043-4 [pii] AID - 101993 [pii] AID - 10.1016/j.jsps.2024.101993 [doi] PST - ppublish SO - Saudi Pharm J. 2024 Mar;32(3):101993. doi: 10.1016/j.jsps.2024.101993. Epub 2024 Feb 12. PMID- 37755516 OWN - NLM STAT- MEDLINE DCOM- 20240214 LR - 20240214 IS - 1432-1912 (Electronic) IS - 0028-1298 (Linking) VI - 397 IP - 3 DP - 2024 Mar TI - Saussurea costus (Falc.) Lipsch.: a comprehensive review of its pharmacology, phytochemicals, ethnobotanical uses, and therapeutic potential. PG - 1505-1524 LID - 10.1007/s00210-023-02694-0 [doi] AB - Saussurea costus (Falc.) Lipsch., commonly known as costus, is a perennial herb that has been traditionally used in various indigenous medicinal systems across Asia. Its historical prominence in traditional remedies underscores the need to explore its phytochemical composition, pharmacological properties, and potential therapeutic benefits. This review aims to provide a comprehensive overview of the available literature on the pharmacological properties, phytochemical constituents, ethnobotanical uses, and therapeutic potential of S. costus. An exhaustive search was performed across multiple electronic databases, including PubMed/MedLine, Google Scholar, Web of Science, Scopus, TRIP database, and Science Direct. Both experimental and clinical studies, as well as traditional ethnobotanical records, were considered for inclusion. The phytochemical analysis revealed that S. costus contains a plethora of bioactive compounds, including sesquiterpenes, flavonoids, and essential oils, which are responsible for its myriad of medicinal properties. The pharmacological studies have demonstrated its anti-inflammatory, anti-oxidant, anti-cancer, hepatoprotective, and immunomodulatory effects, among others. Ethnobotanical data showcased its extensive use in treating ailments like asthma, digestive disorders, and skin conditions. Some clinical trials also underscore its efficacy in certain health conditions, corroborating its traditional uses. S. costus possesses significant therapeutic potential, largely attributable to its rich phytochemical composition; the convergence of its traditional uses and modern pharmacological findings suggests promising avenues for future research, especially in drug development and understanding its mechanism of action in various ailments. CI - © 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Kumari, Ruchika AU - Kumari R AD - Department of Plant Science, School of Life Sciences, Central University of Himachal Pradesh, Dharamshala, Himachal Pradesh, India. FAU - Negi, Madhvi AU - Negi M AD - Department of Plant Science, School of Life Sciences, Central University of Himachal Pradesh, Dharamshala, Himachal Pradesh, India. FAU - Thakur, Palak AU - Thakur P AD - Department of Plant Science, School of Life Sciences, Central University of Himachal Pradesh, Dharamshala, Himachal Pradesh, India. FAU - Mahajan, Himadri AU - Mahajan H AD - Department of Plant Science, School of Life Sciences, Central University of Himachal Pradesh, Dharamshala, Himachal Pradesh, India. FAU - Raina, Kirti AU - Raina K AD - Department of Plant Science, School of Life Sciences, Central University of Himachal Pradesh, Dharamshala, Himachal Pradesh, India. FAU - Sharma, Rohit AU - Sharma R AD - Department of Forest Products, College of Forestry, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, Himachal Pradesh, India. FAU - Singh, Randeep AU - Singh R AD - Department of Zoology, Khalsa College, Amritsar, 143002, Punjab, India. FAU - Anand, Vikas AU - Anand V AD - Department of Physics & Astronomical Sciences, Central University of Himachal Pradesh, Dharamshala, Himachal Pradesh, India. FAU - Ming, Long Chiau AU - Ming LC AD - School of Medical and Life Sciences, Sunway University, 47500, Bandar Sunway, Malaysia. FAU - Goh, Khang Wen AU - Goh KW AD - Faculty of Data Science and Information Technology, INTI International University, 71800, Nilai, Malaysia. FAU - Calina, Daniela AU - Calina D AD - Department of Clinical Pharmacy, University of Medicine and Pharmacy of Craiova, 200349, Craiova, Romania. calinadaniela@gmail.com. FAU - Sharifi-Rad, Javad AU - Sharifi-Rad J AD - Facultad de Medicina, Universidad del Azuay, Cuenca, Ecuador. javad.sharifirad@gmail.com. FAU - Chaudhary, Ashun AU - Chaudhary A AD - Department of Plant Science, School of Life Sciences, Central University of Himachal Pradesh, Dharamshala, Himachal Pradesh, India. ashun.chaudhary@gmail.com. LA - eng PT - Journal Article PT - Review DEP - 20230927 PL - Germany TA - Naunyn Schmiedebergs Arch Pharmacol JT - Naunyn-Schmiedeberg's archives of pharmacology JID - 0326264 RN - 0 (Plant Extracts) RN - 0 (Phytochemicals) RN - 0 (Sesquiterpenes) SB - IM MH - *Saussurea/chemistry MH - Phytotherapy MH - Plant Extracts/pharmacology/therapeutic use/chemistry MH - Phytochemicals/pharmacology/therapeutic use/analysis MH - *Sesquiterpenes/pharmacology OTO - NOTNLM OT - Bioactive phytoconstituents OT - Clinical studies OT - Pharmacological properties OT - Saussurea costus OT - Toxicity EDAT- 2023/09/27 12:41 MHDA- 2024/02/11 16:43 CRDT- 2023/09/27 11:05 PHST- 2023/07/21 00:00 [received] PHST- 2023/08/24 00:00 [accepted] PHST- 2024/02/11 16:43 [medline] PHST- 2023/09/27 12:41 [pubmed] PHST- 2023/09/27 11:05 [entrez] AID - 10.1007/s00210-023-02694-0 [pii] AID - 10.1007/s00210-023-02694-0 [doi] PST - ppublish SO - Naunyn Schmiedebergs Arch Pharmacol. 2024 Mar;397(3):1505-1524. doi: 10.1007/s00210-023-02694-0. Epub 2023 Sep 27. PMID- 38400892 OWN - NLM STAT- MEDLINE DCOM- 20240226 LR - 20240226 IS - 1559-131X (Electronic) IS - 1357-0560 (Linking) VI - 41 IP - 4 DP - 2024 Feb 24 TI - Exploring the Potential of Essential Oil from Plectranthus amboinicus Leaves against Breast Cancer: In vitro and In silico Analysis. PG - 81 LID - 10.1007/s12032-024-02325-5 [doi] AB - Plectranthus amboinicus leaves were subjected to hydrodistillation to obtain essential oil (EO). Phytochemical analysis using gas chromatography-mass spectrometry revealed a diverse range of compounds in the EO, with p-cymen-4-ol (18.57%) emerging as the most predominant, followed by isocaryophyllene (12.18%). The in vitro antiproliferative activity of EO against breast cancer was assessed in MCF-7 and MDA-MB-231 cell lines. The MTT assay results revealed that EO showed IC(50) values of 42.25 μg/mL and 13.44 μg/mL in MCF-7 cells and 63.67 μg/mL and 26.58 μg/mL in MDA-MB-231 cells after 24 and 48 h, respectively. The in silico physicochemical and pharmacokinetic profiles of the EO constituents were within acceptable limits. Molecular docking was conducted to investigate the interactions between the constituents of the EO and protein Aromatase (PDB ID:3S79). Among the EO constituents, 4-tert-butyl-2-(5-tert-butyl-2-hydroxyphenyl)phenol (4BHP) exhibited the highest dock score of -6.580 kcal/mol when compared to the reference drug, Letrozole (-5.694 kcal/mol), but was slightly lesser than Anastrozole (-7.08 kcal/mol). Molecular dynamics simulation studies (100 ns) of the 4BHP complex were performed to study its stability patterns. The RMSD and RMSF values of the 4BHP protein complex were found to be 2.03 Å and 4.46 Å, respectively. The binding free energy calculations revealed that 4BHP displayed the highest negative binding energy of -43 kcal/mol with aromatase protein, compared to Anastrozole (-40.59 kcal/mol) and Letrozole (-44.54 kcal/mol). However, further research is required to determine the safety, efficacy, and mechanism of action of the volatile oil. Taking into consideration the key findings of the present work, the development of a formulation of essential oil remains a challenging task and novel drug delivery systems may lead to site-specific and targeted delivery for the effective treatment of breast cancer. CI - © 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Gupta, Khushi AU - Gupta K AUID- ORCID: 0009-0005-1486-1201 AD - Department of Pharmaceutical Sciences and Natural Products, Central University of Punjab, Ghudda, Bathinda, 151401, Punjab, India. FAU - Gautre, Pranay AU - Gautre P AUID- ORCID: 0009-0008-6363-6432 AD - Department of Pharmaceutical Sciences and Natural Products, Central University of Punjab, Ghudda, Bathinda, 151401, Punjab, India. FAU - Biharee, Avadh AU - Biharee A AUID- ORCID: 0000-0003-0153-2941 AD - Department of Pharmaceutical Sciences and Natural Products, Central University of Punjab, Ghudda, Bathinda, 151401, Punjab, India. FAU - Singh, Yogesh AU - Singh Y AUID- ORCID: 0000-0001-5626-1486 AD - Department of Pharmaceutical Sciences and Natural Products, Central University of Punjab, Ghudda, Bathinda, 151401, Punjab, India. FAU - Patil, Umesh Kumar AU - Patil UK AUID- ORCID: 0000-0002-2096-9922 AD - Department of Pharmaceutical Sciences, Dr. Harisingh Gour Vishwavidyalaya (A Central University), Sagar, 470003, M.P, India. FAU - Kumar, Shashank AU - Kumar S AUID- ORCID: 0000-0002-9622-0512 AD - Molecular Signaling & Drug Discovery Laboratory, Department of Biochemistry, Central University of Punjab, Bathinda, 151 401, Punjab, India. shashankbiochemau@gmail.com. FAU - Thareja, Suresh AU - Thareja S AUID- ORCID: 0000-0001-7668-462X AD - Department of Pharmaceutical Sciences and Natural Products, Central University of Punjab, Ghudda, Bathinda, 151401, Punjab, India. sureshthareja@gmail.com. LA - eng PT - Journal Article DEP - 20240224 PL - United States TA - Med Oncol JT - Medical oncology (Northwood, London, England) JID - 9435512 RN - 0 (Oils, Volatile) RN - EC 1.14.14.1 (Aromatase) RN - 2Z07MYW1AZ (Anastrozole) RN - 7LKK855W8I (Letrozole) SB - IM MH - Humans MH - Female MH - *Oils, Volatile/pharmacology/analysis/chemistry MH - *Plectranthus/chemistry/metabolism MH - Aromatase/metabolism MH - *Breast Neoplasms/drug therapy MH - Anastrozole/metabolism MH - Letrozole/metabolism MH - Molecular Docking Simulation OTO - NOTNLM OT - Breast cancer OT - Essential oil OT - Molecular docking OT - Molecular dynamics simulation OT - Plectranthus amboinicus EDAT- 2024/02/24 21:42 MHDA- 2024/02/26 06:44 CRDT- 2024/02/24 11:05 PHST- 2024/01/03 00:00 [received] PHST- 2024/02/05 00:00 [accepted] PHST- 2024/02/26 06:44 [medline] PHST- 2024/02/24 21:42 [pubmed] PHST- 2024/02/24 11:05 [entrez] AID - 10.1007/s12032-024-02325-5 [pii] AID - 10.1007/s12032-024-02325-5 [doi] PST - epublish SO - Med Oncol. 2024 Feb 24;41(4):81. doi: 10.1007/s12032-024-02325-5. PMID- 38387002 OWN - NLM STAT- Publisher LR - 20240222 IS - 1557-7600 (Electronic) IS - 1096-620X (Linking) DP - 2024 Feb 22 TI - Anticancer Effects of α-Pinene in AGS Gastric Cancer Cells. LID - 10.1089/jmf.2023.K.0267 [doi] AB - Gastric cancer is the fifth most common cancer globally and the third leading cause of cancer-related mortality. Existing treatment strategies for gastric cancer often present numerous side effects. Consequently, recent studies have shifted toward devising new treatments grounded in safer natural substances. α-Pinene, a natural terpene found in the essential oils of various plants, such as Lavender angustifolia and Satureja myrtifolia, displays antioxidant, antibiotic, and anticancer properties. Yet, its impact on gastric cancer remains unexplored. This research assessed the effects of α-pinene in vitro using a human gastric adenocarcinoma cell-line (AGS) human gastric cancer cells and in vivo via a xenograft mouse model. The survival rate of AGS cells treated with α-pinene was notably lower than that of the control group, as revealed by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide assay. This decline in cell viability was linked to apoptosis, as verified by 4',6-diamidino-2-phenylindole and annexin V/propidium iodide staining. The α-pinene-treated group exhibited elevated cleaved-poly (ADP-ribose) polymerase and B cell lymphoma 2 (Bcl-2)-associated X (Bax) levels and reduced Bcl-2 levels compared with the control levels. Moreover, α-pinene triggered the activation of extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 within the mitogen-activated protein kinase (MAPK) pathway. In the xenograft mouse model, α-pinene induced apoptosis through the MAPK pathway, devoid of toxicity. These findings position α-pinene as a promising natural therapeutic for gastric cancer. FAU - Han, Eun-Ji AU - Han EJ AD - Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Korea. FAU - Choi, Eun-Young AU - Choi EY AD - Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Korea. FAU - Jeon, Su-Ji AU - Jeon SJ AD - Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Korea. FAU - Moon, Jun-Mo AU - Moon JM AD - Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Korea. FAU - Lee, Sang-Woo AU - Lee SW AD - Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Korea. FAU - Lee, Jae-Han AU - Lee JH AD - Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Korea. FAU - Jung, Gi-Hwan AU - Jung GH AD - Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Korea. FAU - Han, So-Hee AU - Han SH AD - Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Korea. FAU - Jung, Soo-Hyun AU - Jung SH AD - Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Korea. FAU - Yang, Myeon-Sik AU - Yang MS AD - Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Korea. FAU - Jung, Ji-Youn AU - Jung JY AD - Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Korea. AD - Research Institute for Natural Products, Kongju National University, Yesan, Korea. AD - Research Center of Crop Breeding for Omics and Artificial Intelligence, Yesan, Korea. LA - eng PT - Journal Article DEP - 20240222 PL - United States TA - J Med Food JT - Journal of medicinal food JID - 9812512 SB - IM OTO - NOTNLM OT - MAPK pathway OT - anticancer effects OT - apoptosis OT - gastric cancer OT - xenograft mouse model OT - α-pinene EDAT- 2024/02/22 18:43 MHDA- 2024/02/22 18:43 CRDT- 2024/02/22 16:34 PHST- 2024/02/22 18:43 [medline] PHST- 2024/02/22 18:43 [pubmed] PHST- 2024/02/22 16:34 [entrez] AID - 10.1089/jmf.2023.K.0267 [doi] PST - aheadofprint SO - J Med Food. 2024 Feb 22. doi: 10.1089/jmf.2023.K.0267. PMID- 38365676 OWN - NLM STAT- MEDLINE DCOM- 20240219 LR - 20240219 IS - 2662-7671 (Electronic) IS - 2662-7671 (Linking) VI - 24 IP - 1 DP - 2024 Feb 16 TI - Chemical composition, anticancer, antimicrobial activity of Aloysia citriodora Palau essential oils from four different locations in Palestine. PG - 94 LID - 10.1186/s12906-024-04390-9 [doi] LID - 94 AB - The primary aim of this investigation was to determine the anticancer and antimicrobial properties of essential oils (EOs) extracted from the leaves of Aloysia citriodora Palau, which were procured from four separate locations in Palestine, in addition to analyzing their chemical composition. These areas include Jericho, which has the distinction of being the lowest location on Earth, at 260 m below sea level. The EOs were acquired by hydrodistillation, and their chemical composition was examined utilizing gas chromatography-mass spectrometry (GC-MS). The minimum inhibitory concentration (MIC) of EOs was assessed against six bacterial strains and one fungal species using 96-well microtiter plates. The primary components found in these oils are geranial (26.32-37.22%), neral (18.38-29.00%), and α-curcumene (7.76-16.91%) in three regions. α-Curcumene (26.94%), spathulenol (13.69%), geranial (10.79%), caryophyllene oxide (8.66%), and neral (7.59%) were found to be the most common of the 32 chemical components in the EO from Jericho. The EOs exhibited bactericidal properties, particularly against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), and showed highly effective fungicidal activity. Nevertheless, the antifungal efficacy of the EO was found to surpass its antibacterial activity when administered at lower dosages. The EOs exhibited anticancer activities against melanoma cancer cells, as indicated by their IC(50) values, which ranged from 4.65 to 7.96 μg/mL. A. citriodora EO possesses substantial antifungal and anticancer characteristics, rendering it appropriate for utilization in food-related contexts, hence potentially enhancing the sustainability of the food sector. CI - © 2024. The Author(s). FAU - Al-Maharik, Nawaf AU - Al-Maharik N AD - Department of Chemistry, Faculty of Sciences, An-Najah National University, Nablus P.O. Box. 7, Nablus, 99900800, Palestine. n.maharik@najah.edu. FAU - Salama, Yousef AU - Salama Y AD - An-Najah Center for Cancer and Stem Cell Research, Faculty of Medicine and Health Sciences, An-Najah National University, P.O. Box 7, Nablus, 00970, Palestine. FAU - Al-Hajj, Nisreen AU - Al-Hajj N AD - Department of Chemistry, Faculty of Sciences, An-Najah National University, Nablus P.O. Box. 7, Nablus, 99900800, Palestine. FAU - Jaradat, Nidal AU - Jaradat N AD - Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, P.O. Box. 7, Palestine. FAU - Jobran, Naji Thaer AU - Jobran NT AD - Department of Chemistry, Faculty of Sciences, Birzeit University, Birzeit, P.O. Box. 7, Palestine. FAU - Warad, Ismael AU - Warad I AD - Department of Chemistry, Faculty of Sciences, An-Najah National University, Nablus P.O. Box. 7, Nablus, 99900800, Palestine. FAU - Hamdan, Lina AU - Hamdan L AD - Department of Chemistry, Faculty of Sciences, An-Najah National University, Nablus P.O. Box. 7, Nablus, 99900800, Palestine. FAU - Alrob, Moataz Abo AU - Alrob MA AD - Department of Chemistry, Faculty of Sciences, An-Najah National University, Nablus P.O. Box. 7, Nablus, 99900800, Palestine. FAU - Sawafta, Asil AU - Sawafta A AD - An-Najah Center for Cancer and Stem Cell Research, Faculty of Medicine and Health Sciences, An-Najah National University, P.O. Box 7, Nablus, 00970, Palestine. FAU - Hidmi, Adel AU - Hidmi A AD - Department of Chemistry, Faculty of Sciences, Birzeit University, Birzeit, P.O. Box. 7, Palestine. LA - eng PT - Journal Article DEP - 20240216 PL - England TA - BMC Complement Med Ther JT - BMC complementary medicine and therapies JID - 101761232 RN - 0 (Oils, Volatile) RN - T7EU0O9VPP (citral) RN - 644-30-4 (alpha-curcumene) RN - 0 (Antifungal Agents) RN - 0 (Anti-Infective Agents) RN - 0 (Anti-Bacterial Agents) RN - 0 (Acyclic Monoterpenes) RN - 0 (Sesquiterpenes) RN - Aloysia SB - IM MH - *Oils, Volatile/pharmacology/chemistry MH - Antifungal Agents/pharmacology/chemistry MH - *Methicillin-Resistant Staphylococcus aureus MH - Palau MH - *Anti-Infective Agents/pharmacology MH - Anti-Bacterial Agents/pharmacology/chemistry MH - *Acyclic Monoterpenes MH - *Sesquiterpenes MH - *Verbenaceae PMC - PMC10870676 OTO - NOTNLM OT - Aloysia citriodora OT - Antibacterial OT - Antifungal OT - Colorectal cancer OT - Phytochemicals COIS- The authors declare no competing interests. EDAT- 2024/02/17 10:42 MHDA- 2024/02/19 06:43 CRDT- 2024/02/16 23:36 PHST- 2023/12/01 00:00 [received] PHST- 2024/02/05 00:00 [accepted] PHST- 2024/02/19 06:43 [medline] PHST- 2024/02/17 10:42 [pubmed] PHST- 2024/02/16 23:36 [entrez] AID - 10.1186/s12906-024-04390-9 [pii] AID - 4390 [pii] AID - 10.1186/s12906-024-04390-9 [doi] PST - epublish SO - BMC Complement Med Ther. 2024 Feb 16;24(1):94. doi: 10.1186/s12906-024-04390-9. PMID- 38353827 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20240217 IS - 2731-9229 (Electronic) IS - 2731-9229 (Linking) VI - 19 IP - 1 DP - 2024 Feb 14 TI - Synergistic anticancer effect of Pistacia lentiscus essential oils and 5-Fluorouracil co-loaded onto biodegradable nanofibers against melanoma and breast cancer. PG - 27 LID - 10.1186/s11671-024-03962-5 [doi] LID - 27 AB - Chemoresistance and severe toxicities represent major drawbacks of chemotherapy. Natural extracts, including the essential oils of Pistacia lentiscus (PLEO), exhibit substantial anticancer and anti-inflammatory activities where different cancers are reported to dramatically recess following targeting with PLEO. PLEO has promising antimicrobial, anticancer, and anti-inflammatory properties. However, the therapeutic properties of PLEO are restricted by limited stability, bioavailability, and targeting ability. PLEO nanoformulation can maximize their physicochemical and therapeutic properties, overcoming their shortcomings. Hence, PLEO was extracted and its chemical composition was determined by GC-MS. PLEO and 5-Fluorouracil (5FU) were electrospun into poly-ε-caprolactone nanofibers (PCL-NFs), of 290.71 nm to 680.95 nm diameter, to investigate their anticancer and potential synergistic activities against triple-negative breast cancer cells (MDA-MB-231), human adenocarcinoma breast cancer cells (MCF-7), and human skin melanoma cell line (A375). The prepared nanofibers (NFs) showed enhanced thermal stability and remarkable physical integrity and tensile strength. Biodegradability studies showed prolonged stability over 42 days, supporting the NFs use as a localized therapy of breast tissues (postmastectomy) or melanoma. Release studies revealed sustainable release behaviors over 168 h, with higher released amounts of 5FU and PLEO at pH 5.4, indicating higher targeting abilities towards cancer tissues. NFs loaded with PLEO showed strong antioxidant properties. Finally, NFs loaded with either PLEO or 5FU depicted greater anticancer activities compared to free compounds. The highest anticancer activities were observed with NFs co-loaded with PLEO and 5FU. The developed 5FU-PLEO-PCL-NFs hold potential as a local treatment of breast cancer tissues (post-mastectomy) and melanoma to minimize their  possible recurrence. CI - © 2024. The Author(s). FAU - Alabrahim, Obaydah Abd Alkader AU - Alabrahim OAA AD - Department of Chemistry, School of Sciences & Engineering, The American University in Cairo, AUC Avenue, SSE # 1184, P.O. Box 74, New Cairo, 11835, Egypt. FAU - Azzazy, Hassan Mohamed El-Said AU - Azzazy HME AD - Department of Chemistry, School of Sciences & Engineering, The American University in Cairo, AUC Avenue, SSE # 1184, P.O. Box 74, New Cairo, 11835, Egypt. hazzazy@aucegypt.edu. AD - Department of Nanobiophotonics, Leibniz Institute of Photonic Technology, Albert Einstein Str. 9, Jena, Germany. hazzazy@aucegypt.edu. LA - eng PT - Journal Article DEP - 20240214 PL - Switzerland TA - Discov Nano JT - Discover nano JID - 9918540788706676 PMC - PMC10866856 OTO - NOTNLM OT - 5-Fluorouracil OT - Breast cancer OT - Chemotherapeutics OT - Drug delivery OT - Essential oils OT - Melanoma OT - Nanofibers OT - Natural extracts OT - Pistacia lentiscus OT - Synergistic anticancer activity COIS- The authors declare no competing interests. EDAT- 2024/02/14 12:43 MHDA- 2024/02/14 12:44 CRDT- 2024/02/14 11:10 PHST- 2023/12/07 00:00 [received] PHST- 2024/01/22 00:00 [accepted] PHST- 2024/02/14 12:44 [medline] PHST- 2024/02/14 12:43 [pubmed] PHST- 2024/02/14 11:10 [entrez] AID - 10.1186/s11671-024-03962-5 [pii] AID - 3962 [pii] AID - 10.1186/s11671-024-03962-5 [doi] PST - epublish SO - Discov Nano. 2024 Feb 14;19(1):27. doi: 10.1186/s11671-024-03962-5. PMID- 38359649 OWN - NLM STAT- Publisher LR - 20240215 IS - 1532-2661 (Electronic) IS - 0034-5288 (Linking) VI - 170 DP - 2024 Feb 12 TI - A meta-analysis of essential oils as a dietary additive for weaned piglets: Growth performance, antioxidant status, immune response, and intestinal morphology. PG - 105181 LID - S0034-5288(24)00047-X [pii] LID - 10.1016/j.rvsc.2024.105181 [doi] AB - This study aimed to evaluate the effects of dietary supplementation with EOS on growth performance, blood serum antioxidant status, immune response, and intestinal morphology of weaned piglets using a meta-analytical approach. The database included 31 studies from which the response variables of interest were obtained. All data were analyzed using a random effects model, and results were expressed as weighted mean differences between treatments supplemented with and without EOS. EOS supplementation increased (P < 0.001) average daily feed intake, average daily gain, and final body weight and decreased (P < 0.001) feed conversion ratio and diarrhea incidence. Lower (P = 0.001) serum malondialdehyde content and higher (P < 0.05) serum concentrations of superoxide dismutase, catalase, glutathione peroxidase, and total antioxidant capacity were observed in response to the dietary inclusion of EOS. EOS supplementation increased (P < 0.001) the serum concentration of immunoglobulins A, G, and M and decreased (P < 0.05) the serum concentration of tumor necrosis factor-α, interleukin-1β, and interleukin-6. Greater (P ≤ 0.001) villus height (VH) was observed in the jejunum and ileum in response to the dietary inclusion of EOS. However, EOS supplementation did not affect (P > 0.05) crypt depth (CD) and decreased (P < 0.001) the VH/CD ratio in the duodenum, jejunum, and ileum. In conclusion, essential oils can be used as a dietary additive to improve growth performance and reduce the incidence of diarrhea in weaned piglets and, at the same time, improve the antioxidant status in blood serum, immune response, and intestinal morphology. CI - Copyright © 2024 Elsevier Ltd. All rights reserved. FAU - Hernández-García, Pedro Abel AU - Hernández-García PA AD - Centro Universitario UAEM Amecameca, Universidad Autónoma del Estado de México, Amecameca, Mexico. FAU - Orzuna-Orzuna, José Felipe AU - Orzuna-Orzuna JF AD - Departamento de Zootecnia, Universidad Autónoma Chapingo, Texcoco, Mexico. Electronic address: jforzuna@gmail.com. FAU - Godina-Rodríguez, Juan Eduardo AU - Godina-Rodríguez JE AD - Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Campo Experimental Uruapan, Av. Latinoamérica 1001, Uruapan, Michoacán C.P. 60150, Mexico. FAU - Chay-Canul, Alfonso Juventino AU - Chay-Canul AJ AD - División Académica de Ciencias Agropecuarias, Universidad Juárez Autónoma de Tabasco, Villahermosa, Mexico. FAU - Silva, Gabriela Vázquez AU - Silva GV AD - Departamento El Hombre y su Ambiente, Universidad Autónoma Metropolitana-Xochimilco, Coyoacán, Ciudad de México, Mexico. LA - eng PT - Journal Article DEP - 20240212 PL - England TA - Res Vet Sci JT - Research in veterinary science JID - 0401300 SB - IM OTO - NOTNLM OT - Bioactive compounds OT - Diarrhea incidence OT - Meta-regression OT - Nutrient digestibility OT - Phytogenics COIS- Declaration of competing interest The authors declare that they have no competing interests. EDAT- 2024/02/16 00:42 MHDA- 2024/02/16 00:42 CRDT- 2024/02/15 18:10 PHST- 2023/12/20 00:00 [received] PHST- 2024/02/07 00:00 [revised] PHST- 2024/02/10 00:00 [accepted] PHST- 2024/02/16 00:42 [medline] PHST- 2024/02/16 00:42 [pubmed] PHST- 2024/02/15 18:10 [entrez] AID - S0034-5288(24)00047-X [pii] AID - 10.1016/j.rvsc.2024.105181 [doi] PST - aheadofprint SO - Res Vet Sci. 2024 Feb 12;170:105181. doi: 10.1016/j.rvsc.2024.105181. PMID- 38399856 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20240224 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 16 IP - 4 DP - 2024 Feb 8 TI - Chitosan-Based Nanoencapsulated Essential Oils: Potential Leads against Breast Cancer Cells in Preclinical Studies. LID - 10.3390/polym16040478 [doi] LID - 478 AB - Since ancient times, essential oils (EOs) derived from aromatic plants have played a significant role in promoting human health. EOs are widely used in biomedical applications due to their medicinal properties. EOs and their constituents have been extensively studied for treating various health-related disorders, including cancer. Nonetheless, their biomedical applications are limited due to several drawbacks. Recent advances in nanotechnology offer the potential for utilising EO-loaded nanoparticles in the treatment of various diseases. In this aspect, chitosan (CS) appears as an exceptional encapsulating agent owing to its beneficial attributes. This review highlights the use of bioactive EOs and their constituents against breast cancer cells. Challenges associated with the use of EOs in biomedical applications are addressed. Essential information on the benefits of CS as an encapsulant, the advantages of nanoencapsulated EOs, and the cytotoxic actions of CS-based nanoencapsulated EOs against breast cancer cells is emphasised. Overall, the nanodelivery of bioactive EOs employing polymeric CS represents a promising avenue against breast cancer cells in preclinical studies. FAU - Tan, Wen-Nee AU - Tan WN AUID- ORCID: 0000-0002-7812-3946 AD - Chemistry Section, School of Distance Education, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia. FAU - Samling, Benedict Anak AU - Samling BA AUID- ORCID: 0000-0002-8920-3968 AD - Chemistry Section, School of Distance Education, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia. AD - Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, Kota Samarahan 94300, Sarawak, Malaysia. FAU - Tong, Woei-Yenn AU - Tong WY AD - Institute of Medical Science Technology, Universiti Kuala Lumpur, Kajang 43000, Selangor, Malaysia. FAU - Chear, Nelson Jeng-Yeou AU - Chear NJ AUID- ORCID: 0000-0001-5050-8230 AD - Centre for Drug Research, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia. FAU - Yusof, Siti R AU - Yusof SR AUID- ORCID: 0000-0003-2257-9933 AD - Centre for Drug Research, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia. FAU - Lim, Jun-Wei AU - Lim JW AUID- ORCID: 0000-0003-0158-8822 AD - HICoE-Centre for Biofuel and Biochemical Research, Institute of Self-Sustainable Building, Department of Fundamental and Applied Sciences, Universiti Teknologi PETRONAS, Seri Iskandar 32610, Perak Darul Ridzuan, Malaysia. AD - Department of Biotechnology, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai 602105, India. FAU - Tchamgoue, Joseph AU - Tchamgoue J AUID- ORCID: 0000-0002-0506-9678 AD - Department of Organic Chemistry, Faculty of Science, University of Yaoundé I, Yaoundé P.O. Box 812, Cameroon. FAU - Leong, Chean-Ring AU - Leong CR AUID- ORCID: 0000-0002-2692-0097 AD - Branch Campus Malaysian Institute of Chemical and Bioengineering Technology, Universiti Kuala Lumpur, Alor Gajah 78000, Melaka, Malaysia. FAU - Ramanathan, Surash AU - Ramanathan S AD - Centre for Drug Research, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia. LA - eng GR - FRGS/1/2023/STG01/USM/02/14/Ministry of Higher Education/ GR - RSC Research Fund Grant (R21-3154732300)/Royal Society of Chemistry/ PT - Journal Article PT - Review DEP - 20240208 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC10891598 OTO - NOTNLM OT - breast cancer OT - chitosan OT - drug delivery OT - nanoencapsulated essential oils OT - therapeutics COIS- The authors declare no conflicts of interest. EDAT- 2024/02/24 11:44 MHDA- 2024/02/24 11:45 CRDT- 2024/02/24 01:21 PHST- 2023/12/26 00:00 [received] PHST- 2024/01/31 00:00 [revised] PHST- 2024/02/05 00:00 [accepted] PHST- 2024/02/24 11:45 [medline] PHST- 2024/02/24 11:44 [pubmed] PHST- 2024/02/24 01:21 [entrez] AID - polym16040478 [pii] AID - polymers-16-00478 [pii] AID - 10.3390/polym16040478 [doi] PST - epublish SO - Polymers (Basel). 2024 Feb 8;16(4):478. doi: 10.3390/polym16040478. PMID- 38233115 OWN - NLM STAT- MEDLINE DCOM- 20240206 LR - 20240206 IS - 1347-3352 (Electronic) IS - 1345-8957 (Linking) VI - 73 IP - 2 DP - 2024 Feb 2 TI - Haplophyllum tuberculatum (Forssk.) A. Juss Essential Oils: Seasonal Contents Variation, Bioactivity of the Traditionally-favored, High-yield and Frequent-use Summer Season Oil. PG - 263-273 LID - 10.5650/jos.ess23055 [doi] AB - Haplophyllum tuberculatum (Forssk.) A.Juss. volatile oils were obtained by distillation of the aerial parts of the plant growing in Libya during the summer and spring seasons. A yield and componential analysis revealed that the summer season oil, which is frequently used in traditional medicaments by North African communities, was high in yield (0.858%) compared to the spring season oil (0.47%), and distinguished by the presence of major and various diverse constituents, some of which are considered chemical markers. Owing to the traditional and high incidence of use of the summer-produced essential oil for the treatment of several disorders, including hepatic diseases, and fatigue, the oil was pharmacologically investigated for its varied bioactivities of anti-microbial, in vivo anti-oxidant, and in vitro anti-cancer properties. Thirty-three compounds were identified and represented 96.2% of the peaks in the GCchromatogram of the summer oil, in which the major volatile constituents were δ-3-carene (21.5%), bornyl acetate (16.9%), and limonene aldehyde (15.2%). The summer-based essential oil of the plant demonstrated moderate anti-bacterial activity against Gram-positive bacteria and a relatively strong antibacterial effect against Gram-negative bacteria as compared to the positive antibacterial controls, ampicillin and gentamicin, respectively. Also, antifungal activity against Aspergillus sp. was observed. The summerproduced oil also exhibited in vivo antioxidant and in vitro anti-cancer activities. FAU - Elshibani, Fatma AU - Elshibani F AD - Department of Pharmacognosy, Faculty of Pharmacy, University of Benghazi. AD - Department of Pharmacognosy, Faculty of Pharmacy, Assalam International University. FAU - Alamami, Abdullah AU - Alamami A AD - Department of Basic Medical Sciences, Faculty of Pharmacy, University of Benghazi. FAU - Khan, Riaz AU - Khan R AD - Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University. AD - Manav Rachna International Institute of Research and Study (MRIIRS, formerly Manav Rachna International University, MRIU). FAU - Sulaiman, Ghassan M AU - Sulaiman GM AD - Division of Biotechnology, Department of Applied Sciences, University of Technology. AD - Department of Pharmacognosy and Medicinal Plants, Faculty of Pharmacy, Al-Azhar University. FAU - Mohammed, Hamdoon A AU - Mohammed HA AUID- ORCID: 0000-0003-2896-6790 AD - Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University. AD - Department of Pharmacognosy and Medicinal Plants, Faculty of Pharmacy, Al-Azhar University. LA - eng PT - Journal Article DEP - 20240116 PL - Japan TA - J Oleo Sci JT - Journal of oleo science JID - 101175339 RN - 0 (Oils, Volatile) RN - 0 (Anti-Bacterial Agents) RN - 0 (Antifungal Agents) RN - 0 (Antioxidants) RN - 0 (Plant Oils) SB - IM MH - *Oils, Volatile/chemistry MH - Seasons MH - Anti-Bacterial Agents/chemistry MH - Antifungal Agents MH - Antioxidants/pharmacology/chemistry MH - *Rutaceae/chemistry MH - Microbial Sensitivity Tests MH - Plant Oils/pharmacology/chemistry OTO - NOTNLM OT - GC-MS OT - Haplophyllum tuberculatum OT - antimicrobial activity OT - antioxidant activity OT - cytotoxicity OT - essential oil OT - glutathione level OT - seasonal oil EDAT- 2024/01/18 00:42 MHDA- 2024/02/06 06:43 CRDT- 2024/01/17 21:37 PHST- 2024/02/06 06:43 [medline] PHST- 2024/01/18 00:42 [pubmed] PHST- 2024/01/17 21:37 [entrez] AID - 10.5650/jos.ess23055 [doi] PST - ppublish SO - J Oleo Sci. 2024 Feb 2;73(2):263-273. doi: 10.5650/jos.ess23055. Epub 2024 Jan 16. PMID- 38242042 OWN - NLM STAT- MEDLINE DCOM- 20240208 LR - 20240208 IS - 1950-6007 (Electronic) IS - 0753-3322 (Linking) VI - 171 DP - 2024 Feb TI - Essential oil of Pterocarpus santalinus L. alleviates behavioral impairments in social defeat stress-exposed mice by regulating neurotransmission and neuroinflammation. PG - 116164 LID - S0753-3322(24)00045-3 [pii] LID - 10.1016/j.biopha.2024.116164 [doi] AB - BACKGROUND: Pterocarpus santalinus L. essential oil (PSEO) is traditionally employed for treating fever and mental aberrations. We aim to explore the antidepressant potential of intranasal PSEO in social defeat stress (SDS)-expose mice and identify its mechanisms and components. METHODS: PSEO components were analyzed using gas chromatography-mass spectrometry (GC-MS). C57BL/6 mice underwent a 10-day SDS with intranasal PSEO (10, 20 mg/kg) for 21 days. Efficacy was evaluated through changes in behaviors and serum corticosterone (CORT), hippocampal neurotransmitter, and inflammatory cytokine levels. In vitro effects were examined using primary hippocampal neurons, PC12 and BV2 cells. RESULTS: GC-MS identified 22 volatile compounds in PSEO, and (+)-ledene (16.7%), cedrol (13.5%), and isoaromadendrene epoxide (7.0%) as major components. PSEO (20 mg/kg) significantly reversed SDS-induced social withdrawal, increased open-area explorations in the open field test (OFT) and elevated plus maze (EPM) test, and reduced immobility time in the tail suspension test (TST) and forced swimming test (FST). PSEO downregulated serum CORT and hippocampal interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α levels, while increasing hippocampal gamma-aminobutyric acid (GABA), norepinephrine (NE), and serotonin (5-HT) levels. PSEO (0.1, 1, 10 µg/mL) reduced neurotoxicity and neuroinflammation in PC12 and BV2 cells, respectively. PSEO (10 µg/mL) enhanced glutamic acid decarboxylase 6 (GAD6)- and GABA B receptor 1 (GABABR1)-positive puncta in the hippocampal neurons and FM1-43 fluorescence intensity. CONCLUSION: Intranasal PSEO exhibited antidepressant-like effects on SDS-exposed mice, potentially through modulating stress hormone, neurotransmission, and neuroinflammation. Further investigation into the pharmacokinetics, bioavailability, and mechanisms of (+)-ledene, cedrol, and isoaromadendrene epoxide is needed. CI - Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved. FAU - Nguyen, Ly Thi Huong AU - Nguyen LTH AD - Department of Physiology, Dongguk University College of Korean Medicine, Gyeongju 38066, Republic of Korea; Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, USA. FAU - Nguyen, Nhi Phuc Khanh AU - Nguyen NPK AD - Department of Physiology, Dongguk University College of Korean Medicine, Gyeongju 38066, Republic of Korea. FAU - Tran, Khoa Nguyen AU - Tran KN AD - Department of Physiology, Dongguk University College of Korean Medicine, Gyeongju 38066, Republic of Korea. FAU - Choi, Ho Jin AU - Choi HJ AD - Department of Anatomy, Dongguk University College of Medicine, and Medical Institute of Dongguk University, Gyeongju, Republic of Korea. FAU - Moon, Il Soo AU - Moon IS AD - Department of Anatomy, Dongguk University College of Medicine, Gyeongju 38066, Republic of Korea. FAU - Shin, Heung-Mook AU - Shin HM AD - Department of Physiology, Dongguk University College of Korean Medicine, Gyeongju 38066, Republic of Korea. FAU - Yang, In-Jun AU - Yang IJ AD - Department of Physiology, Dongguk University College of Korean Medicine, Gyeongju 38066, Republic of Korea. Electronic address: injuny@dongguk.ac.kr. LA - eng PT - Journal Article DEP - 20240118 PL - France TA - Biomed Pharmacother JT - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JID - 8213295 RN - 63ZM9703BO (cedrol) RN - 0 (Oils, Volatile) RN - 0 (Antidepressive Agents) RN - W980KJ009P (Corticosterone) RN - 0 (Tumor Necrosis Factor-alpha) RN - 0 (Epoxy Compounds) RN - 0 (Polycyclic Sesquiterpenes) RN - Pterocarpus santalinus SB - IM MH - Mice MH - Animals MH - *Depression/chemically induced MH - *Oils, Volatile/pharmacology MH - Neuroinflammatory Diseases MH - Social Defeat MH - Mice, Inbred C57BL MH - Antidepressive Agents/pharmacology MH - Hippocampus MH - Corticosterone MH - Tumor Necrosis Factor-alpha/metabolism MH - Behavior, Animal MH - Synaptic Transmission MH - Epoxy Compounds/pharmacology MH - Disease Models, Animal MH - *Polycyclic Sesquiterpenes MH - *Pterocarpus OTO - NOTNLM OT - Depression OT - Essential oil OT - Intranasal administration OT - Pterocarpus santalinus L. OT - Red sandalwood OT - Stress resilience COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2024/01/20 05:41 MHDA- 2024/02/08 06:43 CRDT- 2024/01/19 18:15 PHST- 2023/10/24 00:00 [received] PHST- 2024/01/05 00:00 [revised] PHST- 2024/01/11 00:00 [accepted] PHST- 2024/02/08 06:43 [medline] PHST- 2024/01/20 05:41 [pubmed] PHST- 2024/01/19 18:15 [entrez] AID - S0753-3322(24)00045-3 [pii] AID - 10.1016/j.biopha.2024.116164 [doi] PST - ppublish SO - Biomed Pharmacother. 2024 Feb;171:116164. doi: 10.1016/j.biopha.2024.116164. Epub 2024 Jan 18. PMID- 38102850 OWN - NLM STAT- MEDLINE DCOM- 20240215 LR - 20240215 IS - 1099-1573 (Electronic) IS - 0951-418X (Linking) VI - 38 IP - 2 DP - 2024 Feb TI - Monoterpenoids: An upcoming class of therapeutic agents for modulating cancer metastasis. PG - 939-969 LID - 10.1002/ptr.8081 [doi] AB - Monoterpenoids, a sub-class of terpenoids, are secondary metabolites frequently extracted from the essential oils of aromatic plants. Their antitumor properties including antiproliferative, apoptotic, antiangiogenic, and antimetastatic effects along with other biological activities have been the subject of extensive study due to their diverse characteristics. In recent years, numerous investigations have been conducted to understand its potential anticancer impacts, specifically focusing on antiproliferative and apoptotic mechanisms. Metastasis, a malignancy hallmark, can exert either protective or destructive influences on tumor cells. Despite this, the potential antimetastatic and antiangiogenic attributes of monoterpenoids need further exploration. This review focuses on specific monoterpenoids, examining their effects on metastasis and relevant signaling pathways. The monoterpenoids exhibit a high level of complexity as natural products that regulate metastatic proteins through various signaling pathways, including phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin, mitogen-activated protein kinase/extracellular signal-regulated kinase/jun N-terminal kinase, nuclear factor kappa B, vascular endothelial growth factor, and epithelial mesenchymal transition process. Additionally, this review delves into the biosynthesis and classification of monoterpenoids, their potential antitumor impacts on cell lines, the plant sources of monoterpenoids, and the current status of limited clinical trials investigating their efficacy against cancer. Moreover, monoterpenoids depict promising potential in preventing cancer metastasis, however, inadequate clinical trials limit their drug usage. State-of-the-art techniques and technologies are being employed to overcome the challenges of utilizing monoterpenoids as an anticancer agent. CI - © 2023 John Wiley & Sons Ltd. FAU - Mathur, Anurag AU - Mathur A AUID- ORCID: 0009-0008-1040-3715 AD - Bioprospection and Product Development Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India. AD - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India. FAU - Meena, Abha AU - Meena A AUID- ORCID: 0000-0002-1014-1486 AD - Bioprospection and Product Development Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India. AD - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India. FAU - Luqman, Suaib AU - Luqman S AUID- ORCID: 0000-0001-6568-8107 AD - Bioprospection and Product Development Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India. AD - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India. LA - eng GR - HCP-007 Phase II/CSIR-Aroma Mission/ GR - DU2/MLP-11/CSIR-Aroma Mission/ PT - Journal Article PT - Review DEP - 20231215 PL - England TA - Phytother Res JT - Phytotherapy research : PTR JID - 8904486 RN - 0 (Vascular Endothelial Growth Factor A) RN - 0 (Monoterpenes) RN - EC 2.7.1.- (Phosphatidylinositol 3-Kinases) RN - 0 (Antineoplastic Agents) SB - IM MH - Humans MH - Vascular Endothelial Growth Factor A/metabolism MH - Monoterpenes/pharmacology/therapeutic use MH - Phosphatidylinositol 3-Kinases/metabolism MH - *Antineoplastic Agents/pharmacology/therapeutic use MH - *Neoplasms/drug therapy OTO - NOTNLM OT - MMP OT - VEGF OT - angiogenesis OT - cancer OT - metastasis OT - monoterpenoid EDAT- 2023/12/16 11:44 MHDA- 2024/02/15 06:43 CRDT- 2023/12/16 02:02 PHST- 2023/10/28 00:00 [revised] PHST- 2023/08/07 00:00 [received] PHST- 2023/11/14 00:00 [accepted] PHST- 2024/02/15 06:43 [medline] PHST- 2023/12/16 11:44 [pubmed] PHST- 2023/12/16 02:02 [entrez] AID - 10.1002/ptr.8081 [doi] PST - ppublish SO - Phytother Res. 2024 Feb;38(2):939-969. doi: 10.1002/ptr.8081. Epub 2023 Dec 15. PMID- 38010960 OWN - NLM STAT- MEDLINE DCOM- 20240222 LR - 20240222 IS - 1612-1880 (Electronic) IS - 1612-1872 (Linking) VI - 21 IP - 2 DP - 2024 Feb TI - Chemical Characterization, DNA-Damage Protection, Antiproliferative Activity and in Silico Studies of the Essential Oils from Perralderia coronopifolia Coss. PG - e202301535 LID - 10.1002/cbdv.202301535 [doi] AB - In this study, for the first time, we analyzed the chemical composition of essential oils (EOs) steam-distilled from the flowers and leaves of Perralderia coronopifolia by GC-FID/MS. The objective was to explore new anticancer and antioxidant bioactive substances and understand their mechanisms of action through the use of plant-derived natural products. The major chemical components characterizing the EOs were cis-chrysanthenyl acetate 1, 6-oxocyclonerolidol 2, cis-8-acetoxychrysanthenyl acetate 3, and 6α-hydroxycyclonerolidol 4, respectively. Furthermore, the EOs inhibited cell proliferation in HeLa (human cervix carcinoma) and PC3 (human prostate cancer) cells and protected plasmid DNA from oxidative damage caused by UV-photolyzed H(2) O(2) . Employing a molecular docking study, we elucidated the main compounds' inhibition mechanisms. Consequently, the antitumor activity could be related to the inhibitory property of compound 3 against CDC25B phosphatase. The evaluation of ADMET (absorption, distribution, metabolism, excretion, and toxicity) properties and the density functional theory (DFT) calculations of the major compounds, especially compound 3, offer potential insights for designing and developing new cancer drug candidates. In conclusion, our study provides a framework for future research and development in the field by establishing a scientific foundation for the use of Perralderia coronopifolia essential oils as a prospective source of antioxidant and anticancer agents. CI - © 2023 Wiley-VHCA AG, Zurich, Switzerland. FAU - Boussaha, Sara AU - Boussaha S AUID- ORCID: 0000-0002-3662-1154 AD - Unité de Recherche: Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques, Université Frères Mentouri, Constantine 1. Route d'Aïn El Bey, 25017, Constantine, Algérie. AD - Higher National School of Biotechnology Taoufik KHAZNADAR, nouveau Pôle universitaire Ali Mendili, BP. E66, Constantine, 25100, Algeria. FAU - Lassed, Somia AU - Lassed S AUID- ORCID: 0000-0003-1684-880X AD - Département de Microbiologie et Biochimie, Université Mostefa Benboulaid, Batna-2, 05078, Batna, Algérie. FAU - Abdelwahab, Ahmed B AU - Abdelwahab AB AUID- ORCID: 0000-0002-3949-4189 AD - Temisis Therapeutics, 19 avenue de la Forêt de Haye, 54500, Vandœuvre-lès-Nancy, France. FAU - Krid, Adel AU - Krid A AUID- ORCID: 0000-0002-3802-9568 AD - Laboratoire de Physique Mathématique et Subatomique LPMS, Département de Chimie, Université des Frères Mentouri, 25017, Constantine, Algeria. AD - Pharmaceutical Sciences Research Center (CRSP), Ali Mendjli, Constantine, 25000, Algeria. FAU - Altun, Muhammed AU - Altun M AUID- ORCID: 0000-0001-5294-1716 AD - Plant research laboratory, Chemistry Department, Cankiri Karatekin University, Ballica Campus, 18100, Cankiri, Turkey. FAU - Chalard, Pr Pierre AU - Chalard PP AD - Université Clermont Auvergne, CNRS SIGMA Clermont ICC, F-63000, Clermont Ferrand, France. FAU - Chalchat, Pr Jean Claude AU - Chalchat PJC AUID- ORCID: 0000-0001-5292-0309 AD - Association de Valorisation des Huiles Essentielles et des Arômes (AVAHEA), La Laye 7, 63500, Saint Babel, France. FAU - Figueredo, Gilles AU - Figueredo G AD - Laboratoire d'Analyses des Extraits Végétaux et des Arômes (LEXVA Analytique), 460 Rue du Montant, 63110, Beaumont, France. FAU - Zama, Pr Djamila AU - Zama PD AUID- ORCID: 0000-0002-4312-7701 AD - Unité de Recherche: Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques, Université Frères Mentouri, Constantine 1. Route d'Aïn El Bey, 25017, Constantine, Algérie. FAU - Demirtas, Pr Ibrahim AU - Demirtas PI AUID- ORCID: 0000-0001-8946-647X AD - Plant research laboratory, Chemistry Department, Cankiri Karatekin University, Ballica Campus, 18100, Cankiri, Turkey. FAU - Benayache, Pr Samir AU - Benayache PS AUID- ORCID: 0000-0003-2298-2466 AD - Unité de Recherche: Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques, Université Frères Mentouri, Constantine 1. Route d'Aïn El Bey, 25017, Constantine, Algérie. FAU - Benayache, Pr Fadila AU - Benayache PF AUID- ORCID: 0000-0002-2282-6343 AD - Unité de Recherche: Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques, Université Frères Mentouri, Constantine 1. Route d'Aïn El Bey, 25017, Constantine, Algérie. LA - eng PT - Journal Article DEP - 20231205 PL - Switzerland TA - Chem Biodivers JT - Chemistry & biodiversity JID - 101197449 RN - 0 (Oils, Volatile) RN - 0 (Antioxidants) RN - 0 (Antineoplastic Agents) SB - IM MH - Female MH - Humans MH - *Oils, Volatile/chemistry MH - Antioxidants/chemistry MH - Molecular Docking Simulation MH - Prospective Studies MH - Plant Leaves/chemistry MH - *Antineoplastic Agents/pharmacology OTO - NOTNLM OT - Antiproliferative activity OT - DFT and ADMET studies OT - Molecular docking OT - Perralderia coronopifolia OT - Perralderia coronopifolia oils OT - cis-8-acetoxychrysanthenyl acetate EDAT- 2023/11/27 18:44 MHDA- 2024/02/22 06:42 CRDT- 2023/11/27 12:44 PHST- 2023/09/29 00:00 [received] PHST- 2023/11/26 00:00 [accepted] PHST- 2024/02/22 06:42 [medline] PHST- 2023/11/27 18:44 [pubmed] PHST- 2023/11/27 12:44 [entrez] AID - 10.1002/cbdv.202301535 [doi] PST - ppublish SO - Chem Biodivers. 2024 Feb;21(2):e202301535. doi: 10.1002/cbdv.202301535. Epub 2023 Dec 5. PMID- 37919622 OWN - NLM STAT- MEDLINE DCOM- 20240215 LR - 20240215 IS - 1099-1573 (Electronic) IS - 0951-418X (Linking) VI - 38 IP - 2 DP - 2024 Feb TI - Essential oils and their nanoformulations for breast cancer therapy. PG - 556-591 LID - 10.1002/ptr.8054 [doi] AB - Breast Cancer (BC) is the most prevalent type of cancer in the world. Current treatments include surgery, radiation, and chemotherapy but often are associated with high toxicity to normal tissues, chemoresistance, and relapse. Thus, developing novel therapies which could combat these limitations is essential for effective treatment. In this context, phytochemicals are increasingly getting popular due to their safety profile, ability to efficiently target tumors, and circumvent limitations of existing treatments. Essential Oils (EOs) are mixtures of various phytochemicals which have shown potential anticancer activity in preclinical BC models. However, their clinical translation is limited by factors such as high volatility, low stability, and poor solubility. Nanotechnology has facilitated their encapsulation in a variety of nanostructures and proven to overcome these limitations. In this review, we have efficiently summarized the current knowledge on the anticancer effect of EOs and constituents in both in in vitro and in in vivo BC models. Further, we also provide a descriptive account on the potential of nanotechnology in enhancing the anti-BC activity of EOs and their constituents. The papers discussed in this review were selected using the keywords "antiproliferative Essential Oils in breast cancer," "anticancer activity of Essential Oil in breast cancer," and "cytotoxicity of Essential Oils in breast cancer" performed in PubMed and ScienceDirect databases. CI - © 2023 The Authors. Phytotherapy Research published by John Wiley & Sons Ltd. FAU - Thalappil, Muhammed Ashiq AU - Thalappil MA AD - Department of Neuroscience, Biomedicine and Movement Sciences, Section of Biological Chemistry, University of Verona, Verona, Italy. FAU - Singh, Priya AU - Singh P AD - Nanomedicine Laboratory, Institute of Life Sciences, Bhubaneswar, India. FAU - Carcereri de Prati, Alessandra AU - Carcereri de Prati A AD - Department of Neuroscience, Biomedicine and Movement Sciences, Section of Biological Chemistry, University of Verona, Verona, Italy. FAU - Sahoo, Sanjeeb Kumar AU - Sahoo SK AUID- ORCID: 0000-0002-9096-6954 AD - Nanomedicine Laboratory, Institute of Life Sciences, Bhubaneswar, India. FAU - Mariotto, Sofia AU - Mariotto S AUID- ORCID: 0000-0002-3629-435X AD - Department of Neuroscience, Biomedicine and Movement Sciences, Section of Biological Chemistry, University of Verona, Verona, Italy. FAU - Butturini, Elena AU - Butturini E AD - Department of Neuroscience, Biomedicine and Movement Sciences, Section of Biological Chemistry, University of Verona, Verona, Italy. LA - eng GR - Italian Ministry for Research and Education/ PT - Journal Article PT - Review DEP - 20231102 PL - England TA - Phytother Res JT - Phytotherapy research : PTR JID - 8904486 RN - 0 (Oils, Volatile) RN - 0 (Phytochemicals) SB - IM MH - Humans MH - Female MH - *Oils, Volatile/pharmacology/therapeutic use MH - *Breast Neoplasms/drug therapy/pathology MH - Neoplasm Recurrence, Local/drug therapy MH - Phytochemicals/therapeutic use OTO - NOTNLM OT - breast cancer OT - chemosensitization OT - essential oil OT - monoterpenes OT - nanoformulation OT - nanoparticles OT - sesquiterpenes EDAT- 2023/11/03 06:43 MHDA- 2024/02/15 06:43 CRDT- 2023/11/03 00:44 PHST- 2023/09/22 00:00 [revised] PHST- 2023/07/10 00:00 [received] PHST- 2023/10/08 00:00 [accepted] PHST- 2024/02/15 06:43 [medline] PHST- 2023/11/03 06:43 [pubmed] PHST- 2023/11/03 00:44 [entrez] AID - 10.1002/ptr.8054 [doi] PST - ppublish SO - Phytother Res. 2024 Feb;38(2):556-591. doi: 10.1002/ptr.8054. Epub 2023 Nov 2. PMID- 37273096 OWN - NLM STAT- MEDLINE DCOM- 20240214 LR - 20240214 IS - 1559-0291 (Electronic) IS - 0273-2289 (Linking) VI - 196 IP - 2 DP - 2024 Feb TI - Nanoemulsion-Based System as a Novel and Promising Approach for Enhancing the Antimicrobial and Antitumoral Activity of Thymus vulgaris (L.) Oil in Human Hepatocellular Carcinoma Cells. PG - 949-970 LID - 10.1007/s12010-023-04571-1 [doi] AB - The utilisation of medicinal plants and their essential oils is receiving more attention due to the ineffectiveness of current therapeutic methods in the treatment of various cancers and the rising incidence of bacterial antibiotic resistance. Thymol, an active ingredient of Thymus vulgaris, is known to have hepatoprotective, antibacterial, and antioxidant properties. To overcome major obstacles to their usage, such as quick oxidation and high volatility, plant essential oils must be administered through a system to improve the delivery of their active pharmaceutical ingredient. The bioavailability of active substances may be enhanced by the colloidal dispersion nanoemulsion. Therefore, this study aims to derive a comparative evaluation of the thyme oil nanoemulsion formulation and the characterisation of its antibacterial and antitumorigenic activities. A nanoemulsion (NE) with a droplet size of 122.2 ± 1.079 nm was discovered to be stable and mono-dispersed for 4 months and inhibited the growth of B. subtilis, E. coli, P. aeruginosa, and S. aureus. It also displayed antitumorigenic capabilities in HepG2 cells by arresting the cell cycle in the G2/M phase and upregulating the gene expression levels of Bcl-2-associated X protein (Bax), Caspase 3, 8, and 9, as well as a concomitant concentration-dependent decrease in B-cell leukaemia/lymphoma 2 protein (BCL2). Along with an increase in inducible nitric oxide synthase (iNOS) levels, upregulation of the expression levels of the reactive oxygen species (ROS), mitogen-activated protein kinase (MAPK), and endoplasmic reticulum (ER) stress pathways was also seen, indicating of ROS formation in the cancer cells. CI - © 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Nasra, Simran AU - Nasra S AD - Biological and Life Sciences, School of Arts & Sciences, Ahmedabad University, Central Campus, Navrangpura, Ahmedabad, 380009, Gujarat, India. FAU - Meghani, Nikita AU - Meghani N AD - Biological and Life Sciences, School of Arts & Sciences, Ahmedabad University, Central Campus, Navrangpura, Ahmedabad, 380009, Gujarat, India. FAU - Kumar, Ashutosh AU - Kumar A AD - Biological and Life Sciences, School of Arts & Sciences, Ahmedabad University, Central Campus, Navrangpura, Ahmedabad, 380009, Gujarat, India. ashutosh.kumar@ahduni.edu.in. LA - eng PT - Journal Article DEP - 20230605 PL - United States TA - Appl Biochem Biotechnol JT - Applied biochemistry and biotechnology JID - 8208561 RN - 0 (Reactive Oxygen Species) RN - 0 (Anti-Infective Agents) RN - 0 (Oils, Volatile) RN - 0 (Anti-Bacterial Agents) SB - IM MH - Humans MH - *Thymus Plant MH - *Carcinoma, Hepatocellular/drug therapy MH - Staphylococcus aureus MH - Escherichia coli MH - Reactive Oxygen Species MH - *Liver Neoplasms/drug therapy MH - *Anti-Infective Agents/pharmacology MH - *Oils, Volatile/pharmacology MH - Anti-Bacterial Agents/pharmacology OTO - NOTNLM OT - Antimicrobial OT - Hepatoprotective and antitumorigenic OT - Thyme nanoemulsion EDAT- 2023/06/05 13:04 MHDA- 2024/02/14 12:46 CRDT- 2023/06/05 11:10 PHST- 2023/05/24 00:00 [accepted] PHST- 2024/02/14 12:46 [medline] PHST- 2023/06/05 13:04 [pubmed] PHST- 2023/06/05 11:10 [entrez] AID - 10.1007/s12010-023-04571-1 [pii] AID - 10.1007/s12010-023-04571-1 [doi] PST - ppublish SO - Appl Biochem Biotechnol. 2024 Feb;196(2):949-970. doi: 10.1007/s12010-023-04571-1. Epub 2023 Jun 5. PMID- 38293715 OWN - NLM STAT- Publisher LR - 20240131 IS - 1478-6427 (Electronic) IS - 1478-6419 (Linking) DP - 2024 Jan 31 TI - Multifactorial action of lavender and lavandin oils against filamentous fungi. PG - 1-9 LID - 10.1080/14786419.2024.2301741 [doi] AB - AIMS: In this study, five essential oils (EOs) from different species of Lavandula hybrida abrialis, for Lavandula hybrida R.C., Lavandula hybrida 'super A', Lavandula hybrida 'super Z' and Lavandula vera and its hybrids Lavender were evaluated against 26 dust-isolated fungal strains from North Africa. METHODS AND RESULTS: The composition of the different EOs was determined from volume to dry weight. The photochemical analyses were performed via gas chromatography (GC). The cytotoxic effect of five lavender EOs on human epithelial colorectal adenocarcinoma cells (Caco-2) cell line was done. A total of 26 strains of filamentous fungi including Aspergillus spp., Botrytis cinerea, Ceriporia spp., Fusarium spp. and Penicillium glabrum were isolated from sand dust samples via molecular diagnostic tool of PCR. Fungal strains with the lowest minimal lethal concentration (MLC) were Penicillium glabrum, Ceriporia spp. and a strain of Aspergillus spp. CONCLUSIONS: More studies are needed to verify the activity of this EO against more different fungal species, and determine the active ingredients.Significance and impact of study: MIC of the antifungal efficacy relating to EOs was evaluated. The EOs tests showed no cytotoxic effect at very low concentrations, ranging from 0.03% (IC(50) 0.9132 mg/mL) (L. hybrid Abrialis) to 0.001% (IC(50) 1.631 mg/mL) (L. hybrid R.C.). FAU - Donadu, Matthew Gavino AU - Donadu MG AD - Department of Biomedical Science, University of Sassari, Sassari, Italy. AD - Hospital Pharmacy, Giovanni Paolo II Hospital, ASL Gallura, Olbia, Italy. FAU - Ferrari, Marco AU - Ferrari M AD - Department of Biomedical Science, University of Sassari, Sassari, Italy. FAU - Behzadi, Payam AU - Behzadi P AD - Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran. FAU - Trong Le, Nhan AU - Trong Le N AD - Faculty of Pharmacy, Hue University of Medicine and Pharmacy, Hue University, Hue, Vietnam. FAU - Usai, Donatella AU - Usai D AD - Department of Biomedical Science, University of Sassari, Sassari, Italy. FAU - Fiamma, Maura AU - Fiamma M AD - Analysis Laboratory, Hospital 'San Francesco', Nuoro, Italy. FAU - Battah, Basem AU - Battah B AD - Department of Biochemistry and Microbiology, Faculty of Pharmacy, Syrian Private University (SPU), Daraa International Highway, Damascus, Syria. FAU - Barac, Aleksandra AU - Barac A AD - Faculty of Medicine, University of Belgrade, Belgrade, Serbia. AD - Hospital for Infectious and Tropical Diseases, Clinical Center of Serbia, Belgrade, Serbia. FAU - Bellardi, Maria Grazia AU - Bellardi MG AD - Department of agricultural sciences, University of Bologna, Bologna, Italy. FAU - Hoai, Thi Nguyen AU - Hoai TN AD - Faculty of Pharmacy, Hue University of Medicine and Pharmacy, Hue University, Hue, Vietnam. FAU - Mazzarello, Vittorio AU - Mazzarello V AD - Department of Biomedical Science, University of Sassari, Sassari, Italy. FAU - Rubino, Salvatore AU - Rubino S AD - Department of Biomedical Science, University of Sassari, Sassari, Italy. FAU - Cappuccinelli, Piero AU - Cappuccinelli P AD - Department of Biomedical Science, University of Sassari, Sassari, Italy. FAU - Zanetti, Stefania AU - Zanetti S AD - Department of Biomedical Science, University of Sassari, Sassari, Italy. LA - eng PT - Journal Article DEP - 20240131 PL - England TA - Nat Prod Res JT - Natural product research JID - 101167924 SB - IM OTO - NOTNLM OT - Antifungal activity OT - Aspergillus spp. lavender OT - essential oils OT - filamentous fungi OT - lavandin EDAT- 2024/01/31 06:43 MHDA- 2024/01/31 06:43 CRDT- 2024/01/31 04:34 PHST- 2024/01/31 06:43 [medline] PHST- 2024/01/31 06:43 [pubmed] PHST- 2024/01/31 04:34 [entrez] AID - 10.1080/14786419.2024.2301741 [doi] PST - aheadofprint SO - Nat Prod Res. 2024 Jan 31:1-9. doi: 10.1080/14786419.2024.2301741. PMID- 38330531 OWN - NLM STAT- Publisher LR - 20240208 IS - 1873-6947 (Electronic) IS - 1744-3881 (Linking) VI - 55 DP - 2024 Jan 30 TI - Effects of aromatherapy on nausea and vomiting in patients with cancer: A systematic review and meta-analysis of randomized controlled trials. PG - 101838 LID - S1744-3881(24)00011-2 [pii] LID - 10.1016/j.ctcp.2024.101838 [doi] AB - BACKGROUND: and purpose: Aromatherapy offers a low-risk solution for effectively managing common nausea and vomiting in cancer patients. This systematic review and meta-analysis aimed to assess its impact on these symptoms to facilitate practical guidelines establishment. METHODS: PubMed, Web of Science, Cochrane Library, MEDLINE, CINAHL, and Embase were searched for articles published until April 30, 2023. Inclusion criteria were randomized controlled trials (RCTs) on the effect of aromatherapy on nausea and vomiting in patients with cancer (age ≥18 years). The effect size was calculated using standardized mean differences (SMDs) with a random effects model. Subgroup analyses, meta-analysis of variance, and meta-regression were performed using the "meta" package in R version 4.0.2. Heterogeneity was assessed using I(2) statistics. Sensitivity and publication bias analyses were performed; two reviewers independently assessed risk of bias using Cochrane's risk-of-bias tool 2.0. RESULTS: Twenty-five RCTs across 10 articles revealed that aromatherapy reduced overall nausea and vomiting in patients with cancer with significant efficacy (SMD = -0.81, 95 % confidence interval [CI]: -1.11 to -0.52). Furthermore, aromatherapy reduced nausea (SMD = -0.85, 95 % CI: -1.23 to -0.46) and combined nausea and vomiting (SMD = -1.08, 95 % CI: -1.68 to -0.47), but not vomiting alone (SMD = -0.24, 95 % CI: -1.03 to 0.55). Inhalation and massage yielded positive results, especially in chemotherapy-induced cases; peppermint oil was particularly successful. CONCLUSION: Our findings underscore aromatherapy's value in managing cancer treatment-associated nausea and vomiting. Conclusive evidence on aromatherapy-led nausea reduction is lacking due to limited RCTs; research is warranted for robust conclusions. CI - Copyright © 2024 Elsevier Ltd. All rights reserved. FAU - Ahn, Ju Hyun AU - Ahn JH AD - College of Nursing, Kangwon National University, Republic of Korea. FAU - Kim, Myoungsuk AU - Kim M AD - College of Nursing, Kangwon National University, Republic of Korea. Electronic address: cellylife@gmail.com. FAU - Kim, Ri Whaol AU - Kim RW AD - College of Nursing, Kangwon National University, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20240130 PL - England TA - Complement Ther Clin Pract JT - Complementary therapies in clinical practice JID - 101225531 OTO - NOTNLM OT - Aromatherapy OT - Cancer OT - Meta-analysis OT - Nausea OT - Vomiting EDAT- 2024/02/09 00:42 MHDA- 2024/02/09 00:42 CRDT- 2024/02/08 18:01 PHST- 2023/06/03 00:00 [received] PHST- 2024/01/22 00:00 [revised] PHST- 2024/01/23 00:00 [accepted] PHST- 2024/02/09 00:42 [medline] PHST- 2024/02/09 00:42 [pubmed] PHST- 2024/02/08 18:01 [entrez] AID - S1744-3881(24)00011-2 [pii] AID - 10.1016/j.ctcp.2024.101838 [doi] PST - aheadofprint SO - Complement Ther Clin Pract. 2024 Jan 30;55:101838. doi: 10.1016/j.ctcp.2024.101838. PMID- 38298712 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20240202 IS - 2405-8440 (Print) IS - 2405-8440 (Electronic) IS - 2405-8440 (Linking) VI - 10 IP - 2 DP - 2024 Jan 30 TI - Integrating network pharmacology and experimental verification to decipher the multitarget pharmacological mechanism of Cinnamomum zeylanicum essential oil in treating inflammation. PG - e24120 LID - 10.1016/j.heliyon.2024.e24120 [doi] LID - e24120 AB - Inflammatory diseases contribute to more than 50 % of global deaths. Research suggests that network pharmacology can reveal the biological mechanisms underlying inflammatory diseases and drug effects at the molecular level. The aim of the study was to clarify the biological mechanism of Cinnamomum zeylanicum essential oil (CZEO) and predict molecular targets of CZEO against inflammation by employing network pharmacology and in vitro assays. First, the genes related to inflammation were identified from the Genecards and Online Mendelian Inheritance in Man (OMIM) databases. The CZEO targets were obtained from the SwissTargetPrediction and Similarity Ensemble Approach (SEA) database. A total of 1057 CZEO and 526 anti-inflammation targets were obtained. The core hub target of CZEO anti-inflammatory was obtained using the protein-protein interaction network. KEGG pathway analysis suggested CZEO to exert anti-inflammatory effect mainly through Tumor necrosis factor, Toll-like receptor and IL-17 signalling pathway. Molecular docking of active ingredients-core targets interactions was modelled using Pyrx software. Docking and simulation studies revealed benzyl benzoate to exhibit good binding affinity towards IL8 protein. MTT assay revealed CZEO to have non-cytotoxic effect on RAW 264.7 cells. CZEO also inhibited the production of NO, PGE(2), IL-6, IL-1β and TNF-α and promoted the activity of endogenous antioxidant enzymes in LPS-stimulated RAW 264.7 cells. Additionally, CZEO inhibited intracellular ROS generation, NF-kB nuclear translocation and modulated the expression of downstream genes involved in Toll-like receptor signalling pathway. The results deciphered the mechanism of CZEO in treating inflammation and provided a theoretical basis for its clinical application. CI - © 2024 The Author(s). FAU - Mohanty, Debajani AU - Mohanty D AD - Centre for Biotechnology, Siksha O Anusandhan (Deemed to be University), Kalinganagar, Ghatikia, Bhubaneswar-751003, Odisha, India. FAU - Padhee, Sucheesmita AU - Padhee S AD - Centre for Biotechnology, Siksha O Anusandhan (Deemed to be University), Kalinganagar, Ghatikia, Bhubaneswar-751003, Odisha, India. FAU - Sahoo, Chiranjibi AU - Sahoo C AD - Centre for Biotechnology, Siksha O Anusandhan (Deemed to be University), Kalinganagar, Ghatikia, Bhubaneswar-751003, Odisha, India. FAU - Jena, Sudipta AU - Jena S AD - Centre for Biotechnology, Siksha O Anusandhan (Deemed to be University), Kalinganagar, Ghatikia, Bhubaneswar-751003, Odisha, India. FAU - Sahoo, Ambika AU - Sahoo A AD - Centre for Biotechnology, Siksha O Anusandhan (Deemed to be University), Kalinganagar, Ghatikia, Bhubaneswar-751003, Odisha, India. FAU - Chandra Panda, Pratap AU - Chandra Panda P AD - Centre for Biotechnology, Siksha O Anusandhan (Deemed to be University), Kalinganagar, Ghatikia, Bhubaneswar-751003, Odisha, India. FAU - Nayak, Sanghamitra AU - Nayak S AD - Centre for Biotechnology, Siksha O Anusandhan (Deemed to be University), Kalinganagar, Ghatikia, Bhubaneswar-751003, Odisha, India. FAU - Ray, Asit AU - Ray A AD - Centre for Biotechnology, Siksha O Anusandhan (Deemed to be University), Kalinganagar, Ghatikia, Bhubaneswar-751003, Odisha, India. LA - eng PT - Journal Article DEP - 20240110 PL - England TA - Heliyon JT - Heliyon JID - 101672560 PMC - PMC10828654 OTO - NOTNLM OT - Anti-inflammatory OT - Cinnamomum zeylanicum bark essential oil OT - Molecular docking OT - Network pharmacology OT - RAW 264.7 cells COIS- The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:No If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2024/02/01 06:42 MHDA- 2024/02/01 06:43 CRDT- 2024/02/01 04:13 PHST- 2023/07/01 00:00 [received] PHST- 2024/01/03 00:00 [revised] PHST- 2024/01/03 00:00 [accepted] PHST- 2024/02/01 06:43 [medline] PHST- 2024/02/01 06:42 [pubmed] PHST- 2024/02/01 04:13 [entrez] AID - S2405-8440(24)00151-8 [pii] AID - e24120 [pii] AID - 10.1016/j.heliyon.2024.e24120 [doi] PST - epublish SO - Heliyon. 2024 Jan 10;10(2):e24120. doi: 10.1016/j.heliyon.2024.e24120. eCollection 2024 Jan 30. PMID- 38288146 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20240131 IS - 2046-2069 (Electronic) IS - 2046-2069 (Linking) VI - 14 IP - 6 DP - 2024 Jan 23 TI - Innovative microwave-assisted biosynthesis of copper oxide nanoparticles loaded with platinum(ii) based complex for halting colon cancer: cellular, molecular, and computational investigations. PG - 4005-4024 LID - 10.1039/d3ra08779d [doi] AB - In the current study, we biosynthesized copper oxide NPs (CuO NPs) utilizing the essential oils extracted from Boswellia carterii oleogum resin, which served as a bioreductant and capping agent with the help of microwave energy. Afterwards, the platinum(ii) based anticancer drug, carboplatin (Cr), was loaded onto the CuO NPs, exploiting the electrostatic interactions forming Cr@CuO NPs. The produced biogenic NPs were then characterized using zeta potential (ZP), high-resolution transmission electron microscopy (HRTEM), X-ray diffraction spectroscopy (XRD), and Fourier transform infrared spectroscopy (FTIR) techniques. In addition, the entrapment efficiency and release profile of the loaded Cr were evaluated. Thereafter, SRB assay was performed, where Cr@CuO NPs demonstrated the highest cytotoxic activity against human colon cancer cells (HCT-116) with an IC(50) of 5.17 μg mL(-1), which was about 1.6 and 2.2 folds more than that of Cr and CuO NPs. Moreover, the greenly synthesized nanoparticles (Cr@CuO NPs) displayed a satisfactory selectivity index (SI = 6.82), which was far better than the free Cr treatment (SI = 2.23). Regarding the apoptosis assay, the advent of Cr@CuO NPs resulted in an immense increase in the cellular population percentage of HCT-116 cells undergoing both early (16.02%) and late apoptosis (35.66%), significantly surpassing free Cr and CuO NPs. A study of HCT-116 cell cycle kinetics revealed the powerful ability of Cr@CuO NPs to trap cells in the Sub-G1 and G2 phases and impede the G2/M transition. RT-qPCR was utilized for molecular investigations of the pro-apoptotic (Bax and p53) and antiapoptotic genes (Bcl-2). The novel Cr@CuO NPs treatment rose above single Cr or CuO NPs therapy in stimulating the p53-Bax mediated mitochondrial apoptosis. The cellular and molecular biology investigations presented substantial proof of the potentiated anticancer activity of Cr@CuO NPs and the extra benefits that could be obtained from their use. CI - This journal is © The Royal Society of Chemistry. FAU - Sedky, Nada K AU - Sedky NK AUID- ORCID: 0000-0002-2359-3799 AD - Department of Biochemistry, School of Life and Medical Sciences, University of Hertfordshire Hosted by Global Academic Foundation R5 New Garden City, New Administrative Capital Cairo Egypt. FAU - Fawzy, Iten M AU - Fawzy IM AUID- ORCID: 0000-0001-9246-2875 AD - Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Future University in Egypt Cairo 11835 Egypt. FAU - Hassan, Afnan AU - Hassan A AD - Biomedical Sciences Program, Zewail City of Science and Technology Giza 12578 Egypt. FAU - Mahdy, Noha Khalil AU - Mahdy NK AD - Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University Kasr El-Aini Street 11562 Cairo Egypt. FAU - Attia, Reem T AU - Attia RT AD - Department of Pharmacology and Toxicology and Biochemistry, Faculty of Pharmacy, Future University in Egypt Cairo 11835 Egypt. FAU - Shamma, Samir N AU - Shamma SN AD - Institute of Global Health and Human Ecology, School of Sciences & Engineering, The American University in Cairo AUC Avenue, P.O. Box 74 New Cairo 11835 Egypt. FAU - Alfaifi, Mohammad Y AU - Alfaifi MY AD - King Khalid University, Faculty of Science, Biology Department Abha 9004 Saudi Arabia. FAU - Elbehairi, Serag Eldin AU - Elbehairi SE AD - King Khalid University, Faculty of Science, Biology Department Abha 9004 Saudi Arabia. FAU - Mokhtar, Fatma A AU - Mokhtar FA AUID- ORCID: 0000-0002-6909-7440 AD - Department of Pharmacognosy, Faculty of Pharmacy, El Saleheya El Gadida University El Saleheya El Gadida Sharkia 44813 Egypt. FAU - Fahmy, Sherif Ashraf AU - Fahmy SA AUID- ORCID: 0000-0003-3056-8281 AD - Department of Chemistry, School of Life and Medical Sciences, University of Hertfordshire Hosted by Global Academic Foundation R5 New Garden City, New Capital Cairo 11835 Egypt sheriffahmy@aucegypt.edu +20 1222613344. LA - eng PT - Journal Article DEP - 20240129 PL - England TA - RSC Adv JT - RSC advances JID - 101581657 PMC - PMC10823359 COIS- The authors declare no conflict of interest. EDAT- 2024/01/30 06:42 MHDA- 2024/01/30 06:43 CRDT- 2024/01/30 03:38 PHST- 2023/12/22 00:00 [received] PHST- 2024/01/21 00:00 [accepted] PHST- 2024/01/30 06:43 [medline] PHST- 2024/01/30 06:42 [pubmed] PHST- 2024/01/30 03:38 [entrez] AID - d3ra08779d [pii] AID - 10.1039/d3ra08779d [doi] PST - epublish SO - RSC Adv. 2024 Jan 29;14(6):4005-4024. doi: 10.1039/d3ra08779d. eCollection 2024 Jan 23. PMID- 38298493 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20240202 IS - 1565-3633 (Print) IS - 1687-479X (Electronic) VI - 2024 DP - 2024 TI - Retracted: Nanoemulsion and Encapsulation Strategy of Hydrophobic Oregano Essential Oil Increased Human Prostate Cancer Cell Death via Apoptosis by Attenuating Lipid Metabolism. PG - 9831345 LID - 10.1155/2024/9831345 [doi] LID - 9831345 AB - [This retracts the article DOI: 10.1155/2022/9569226.]. CI - Copyright © 2024 Bioinorganic Chemistry and Applications. FAU - And Applications, Bioinorganic Chemistry AU - And Applications BC LA - eng PT - Retraction of Publication DEP - 20240124 PL - Egypt TA - Bioinorg Chem Appl JT - Bioinorganic chemistry and applications JID - 101200445 ROF - Bioinorg Chem Appl. 2022 May 26;2022:9569226. PMID: 35662912 PMC - PMC10830193 EDAT- 2024/02/01 06:43 MHDA- 2024/02/01 06:44 CRDT- 2024/02/01 04:09 PHST- 2024/01/23 00:00 [received] PHST- 2024/01/23 00:00 [accepted] PHST- 2024/02/01 06:44 [medline] PHST- 2024/02/01 06:43 [pubmed] PHST- 2024/02/01 04:09 [entrez] AID - 10.1155/2024/9831345 [doi] PST - epublish SO - Bioinorg Chem Appl. 2024 Jan 24;2024:9831345. doi: 10.1155/2024/9831345. eCollection 2024. PMID- 38276303 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20240129 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 14 IP - 1 DP - 2024 Jan 19 TI - Citrus clementine Peel Essential Oil Ameliorates Potassium Dichromate-Induced Lung Injury: Insights into the PI3K/AKT Pathway. LID - 10.3390/metabo14010068 [doi] LID - 68 AB - Acute Lung Injury (ALI) is a life-threatening syndrome that has been identified as a potential complication of COVID-19. There is a critical need to shed light on the underlying mechanistic pathways and explore novel therapeutic strategies. This study aimed to examine the potential therapeutic effects of Citrus clementine essential oil (CCEO) in treating potassium dichromate (PDC)-induced ALI. The chemical profile of CCEO was created through GC-MS analysis. An in vivo study in rats was conducted to evaluate the effect of CCEO administrated via two different delivery systems (oral/inhalation) in mitigating acute lung injury (ALI) induced by intranasal instillation of PDC. Eight volatile compounds were identified, with monoterpene hydrocarbons accounting for 97.03% of the identified constituents, including 88.84% of D-limonene. CCEO at doses of 100 and 200 mg/kg bw exhibited antioxidant and anti-inflammatory properties. These significant antioxidant properties were revealed through the reduction of malondialdehyde (MDA) and the restoration of reduced glutathione (GSH). In addition, inflammation reduction was observed by decreasing levels of cytokines tumor necrosis factor-α and tumor growth factor-β (TNF-α and TGF-β), along with an increase in phosphatidylinositide-3-kinase (PI3K) and Akt overexpression in lung tissue homogenate, in both oral and inhalation routes, compared to the PDC-induced group. These results were supported by histopathological studies and immunohistochemical assessment of TGF-β levels in lung tissues. These findings revealed that CCEO plays an integral role in relieving ALI induced by intranasal PDC and suggests it as a promising remedy. FAU - Attia, Hany G AU - Attia HG AUID- ORCID: 0000-0002-4494-8309 AD - Department of Pharmacognosy, College of Pharmacy, Najran University, Najran 1988, Saudi Arabia. FAU - El-Morshedy, Suzan M AU - El-Morshedy SM AUID- ORCID: 0009-0002-7489-162X AD - Clinical Pathology Department, National Liver Institute, Menoufia University, Menoufia 32511, Egypt. FAU - Nagy, Ahmed M AU - Nagy AM AUID- ORCID: 0000-0002-0060-4328 AD - Department of Animal Reproduction & AI, Veterinary Research Institute, National Research Center, 33 El Bohouth St., Dokki, Cairo 12622, Egypt. FAU - Ibrahim, Ammar M AU - Ibrahim AM AD - Applied Medical Sciences College, Najran University, Najran 55461, Saudi Arabia. FAU - Aleraky, Mohamed AU - Aleraky M AD - Department of Clinical Pathology, Al-Azhar University, New Damietta 11651, Egypt. FAU - Abdelrahman, Sahar S AU - Abdelrahman SS AD - Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza 12613, Egypt. FAU - Osman, Samir M AU - Osman SM AUID- ORCID: 0000-0001-8275-732X AD - Department of Pharmacognosy, Faculty of Pharmacy, Oct. 6 University, Giza 12585, Egypt. FAU - Alasmari, Saeed M AU - Alasmari SM AD - Applied Medical Sciences College, Najran University, Najran 55461, Saudi Arabia. FAU - El Raey, Mohamed A AU - El Raey MA AUID- ORCID: 0000-0002-5960-3012 AD - Department of Phytochemistry and Plant Systematics, Pharmaceutical Division, National Research Centre, Dokki, Cairo 12622, Egypt. FAU - Abdelhameed, Mohamed F AU - Abdelhameed MF AUID- ORCID: 0000-0001-6100-6498 AD - Pharmacology Department, National Research Centre, 33 El Bohouth St., Dokki, Cairo 12622, Egypt. LA - eng GR - NU/DRP/MRC/12/4/Najran University/ PT - Journal Article DEP - 20240119 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC10818323 OTO - NOTNLM OT - AKT OT - Citrus clementine OT - PI3K OT - lung injury OT - oxidative stress OT - potassium dichromate COIS- The authors declare no conflicts of interest. EDAT- 2024/01/26 12:43 MHDA- 2024/01/26 12:44 CRDT- 2024/01/26 09:26 PHST- 2023/12/21 00:00 [received] PHST- 2024/01/12 00:00 [revised] PHST- 2024/01/16 00:00 [accepted] PHST- 2024/01/26 12:44 [medline] PHST- 2024/01/26 12:43 [pubmed] PHST- 2024/01/26 09:26 [entrez] AID - metabo14010068 [pii] AID - metabolites-14-00068 [pii] AID - 10.3390/metabo14010068 [doi] PST - epublish SO - Metabolites. 2024 Jan 19;14(1):68. doi: 10.3390/metabo14010068.